ABSTRACT
Objective To investigate biological activity and antitumor effects of phosphatidylinositol-3 (GPC3) gene transfected dendritic cells(DC,DC-GPC3)co-cultured with cytokine induced killer cells(CIK)on hepatocellular carcinoma (HCC). Methods The phenotypes of effector cells were analyzed by flow cytometry. Levels of interleukin (IL)-2 and interferon (IFN)–γ were determined by MTT colorimetry and enzyme-linked immunosorbent assay, respectively. The cytotoxic activity against hepatocarcinoma cells (HepG2) was measured by lactate dehydrogenase release. Results The proportions of CD3+CD8+and CD3+CD56+double-positive cells were significantly elevated in the DCIK-GPC3 compared with the DCIK and CIK(P<0.05).Compared with the DCIK and CIK,the DCIK-GPC3 showed significantly higher levels of secreted IL-2 and IFN-γ in the supernatants(P<0.05).The antitumor effect of DCIK-GPC3 against HepG2 was the highest than that of DCIK and CIK at an effector-target ratio ranging from 20:1 to 50:1(P<0.05).Conclusion DCIK-GPC3 can enhance the cytotoxic activity against hepatocarcinoma cells in vitro.This study provides a theoretical and experimental basis for clinical immunotherapy using DCIK-GPC3.
ABSTRACT
Mesenchymal stem cells (MSCs) are defined as undifferentiated cells that are capable of self renewal and functionally capable of differentiating into adipocytes, osteoblasts, chondrocytes, hepatocyte-like cells, myogenic-like cells, neuron-like cells, and islet-like cells. MSCs possess immunomodulatory properties according to secrete indoleamine-2, 3-dioxygenase (IDO) and prostaglandin E2 (PGE2) to play the role of immunomodulatory. Moreover, pre-stimulation of interferon-γ (IFN-γ) can promote the immunomodulatory function of MSCs. High abundance of MSCs found in adipose tissue makes it a very attractive source of adult stem cells. This article reviews the recent progress in the interaction between adipose derived mesenchymal stem cells (ADSCs) and three immunomodulatory factors.